Wham is made for paired-conclude Illumina libraries with common insert dimensions (~300bp-500bp). Wham integrates mate-pair mapping, split browse mapping, soft-clipping, option alignment and consensus sequence based proof to forecast SV breakpoints with one-nucleotide accuracy. Wham generates a blended pileup (catalog of reads masking a situation w
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The tender-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence employing a several sequence alignment (MSA) furnished from the seqAn library [27]. Wham applies three filters on the consensus sequences. Breakpoints aren't described in conditions where by consensus sequences are shorter than ten bp or incorpor